Purchased research samples
I obtained Alzheimer's disease research material through Sanguine Bio for exploratory cell work. The specific product, donor identifiers, and lot information are not in the available project record.
NEURODEGENERATION · COMPLETED PILOT WORK
It started with samples
in culture.
I purchased Alzheimer's disease research material through Sanguine Bio, cultured it using an incubator, and completed exploratory cell-culture tests. That bench work came before the recovery model and the separate ALS study I am now seeking to fund.
SAMPLES PURCHASEDINCUBATOR CULTURE COMPLETEDPILOT TESTS COMPLETED
WHAT I ACTUALLY DID
The pilot happened at the bench.
These are the steps I can account for from the early Alzheimer's-related work. They are not an ALS experiment.
Used an incubator
I carried out incubator-based culture work with the purchased material. The original culture schedule, media, and handling details are not available to publish here.
Completed exploratory tests
I performed pilot cell-culture tests. The assay identity, controls, and raw readouts have not been recovered, so I am not assigning a direction or size to a biological effect.
WHAT I BUILT FROM THE QUESTION
I turned recovery time into a testable model.
After the pilot, I developed a separate hypothesis about how the time between two stress events might change a later cell response. The original example concerned protein seeding in a Parkinson's-related model. It was conceptual work, not an outcome measured in the Alzheimer's samples.
MODEL DRAFT · NOT FITTED TO PILOT DATA
Reaction, clearance, and spread
∂P/∂t = D∇²P + αP(1 − P/K) − μP + I(x,t)I sketched this reaction–diffusion model to make the idea precise. It treats a selected protein signal as something that can spread, amplify, and clear while an experimental pulse adds new input. No parameter in this equation was estimated from the completed culture pilot.
Pprotein signal in a future assay
Dspatial spread, requiring spatial images
αlocal amplification in the model
μclearance of the signal
I(x,t)timed experimental input
The prediction I wrote down:
With the same total challenge, a shorter recovery interval could leave more residual signal and change the response to a second pulse. The proposed comparison would need matched cultures and repeated measurements. The pilot did not test or validate that prediction.
NEXT EXPERIMENT · ALS
The ALS test is a separate next step.
I have since framed an ALS-specific question: could the way a motor neuron recovers after a brief stress predict its response to a second challenge better than a single snapshot? This question grew from the recovery idea, but it requires its own cell model, TDP-43 readout, controls, and independent validation.
I have not run that ALS comparison or measured its predicted effect. The funding plan starts with an assay feasibility step before any human ALS cell comparison.
PROPOSED · NOT A PILOT RESULT
The proposed study would first establish a reliable recovery readout in motor-neuron-like culture, then test it in matched human ALS and control motor neurons. A held-out comparison would ask whether a recovery curve adds predictive information beyond baseline localization alone.
See the study and funding plan